Outcomes

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Publications view all
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Measurement, 122366 (2026)
Cross-instrument measurement framework linking X-ray μCT and photoluminescence spectroscopy: Application to microcracks characterization in mineralized enamel
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Correlative characterization of micro-scale defects in heterogeneous mineralized materials requires the spatial fusion of measurement modalities that differ in resolution, contrast mechanism, and coordinate system. This study presents a non-destructive multimodal measurement methodology in which X-ray micro-computed tomography (µCT) and spatially selective photoluminescence (PL) spectroscopy are linked on an intact specimen by a 3D surface mesh-assisted spatial registration framework, with complementary energy-dispersive X-ray spectroscopy (EDS) used to characterize the associated compositional changes. The registration aligns the volumetric and point-resolved measurements with a spatial uncertainty bounded by the PL laser spot size (diameter 80 µm) and the µCT voxel size (7 × 7 × 7 µm3), both smaller than the spacing between adjacent measurement points, ensuring well-defined co-localization of crack-affected and pristine regions. The methodology was demonstrated on extracted human premolars with visible enamel microcracks: 192 polarization-resolved PL spectra per tooth, acquired at 325 nm excitation across 12 spatial positions (6 cracked, 6 pristine) and spatially registered to the µCT datacube, revealed a reproducible difference in spectral shape between crack-affected and pristine regions. A peak asymmetry index, defined as the ratio of the two emission maxima, was reduced in cracked regions consistently in both teeth and significantly when pooled across specimens (1.16 vs 1.28; p = 0.014), supported by EDS evidence of increased carbon content at crack sites (a 42% relative increase in averaged carbon content, with the carbon-to-oxygen ratio rising from 0.46 to 0.84). The validated measurement framework — its spatial registration with bounded uncertainty — is material-independent and generalizable to correlative volumetric and spectroscopic defect characterization in heterogeneous solids.
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Small Methods (2026): e02420
Analytical Ultracentrifugation in Different High-Density Media Allows to Assess Heterogeneity of mRNA-Lipid Nanoparticles
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This study demonstrates the capability of analytical ultracentrifugation for assessing the morphological homogeneity of mRNA-lipid nanoparticles. Building on a standard method for density evaluation of nanoparticles, it demonstrates the importance of selecting the appropriate liquid medium for the experiments. Our research reveals the interaction between deuterated buffers and aqueous compartments of lipid-based particles. By investigating the sedimentation behavior of compact solid particles, liposomes, and lipid nanoparticles, we demonstrate that AUC can detect water-loaded cavities in mRNA-loaded lipid nanoparticles. Therefore, we suggest that performing sedimentation velocity experiments both in sucrose-containing buffer and deuterated solvents results in precious information not only on density but also on batch homogeneity and particle morphology.
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Photonics 2026, 13(6), 586
Phase Shift Effects in Chiral Plasmonic Nanohole Arrays
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The interaction between light and chiral plasmonic metasurfaces provides a powerful mechanism for controlling polarization states at the nanoscale. Utilizing displacement Talbot lithography for large-area fabrication, we characterized the chiroptical response by measuring the evolution of Stokes parameters to quantify phase retardation between orthogonal polarization components. To elucidate the underlying physical mechanism, we employ a hybrid finite element method and rigorous coupled-wave analysis approach to investigate the behavior of the far-field and local-field configurations. Our results reveal that the phase shift is highly sensitive to symmetry-breaking features, where the interplay between different modes dictates the overall circular dichroism signal. Furthermore, the analysis of local field plots suggests specific contributions of plasmonic modes to the chiroptical response. We conclude that the phase shift effects, characterized via Stokes parameters and modal analysis, provide a robust metric for engineering chiroptical properties in these systems. This work establishes a fundamental framework for developing compact polarization-control elements and enhances the understanding of phase-modulated light-matter interactions in chiral plasmonic metasurfaces.
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Deliverables view all
WP2 - MGT2 - Pilot scheme for the management of a distributed research infrastructure offering harmonised, interoperable and integrated services
D2.9 - Schemes for a sustainable technical and scientific long-term management
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The Integrated Distributed Research Infrastructure for Nanoscience (IDRIN) has been conceived as a coordinated and interoperable operational model for a distributed research infrastructure bringing together the facilities and services offered by the NEP consortium under a common coordinated framework. The NFFA-Europe IDRIN encompasses a broad ensemble of access providers, including both beneficiaries and Third Parties providing access against Payment (TPaP), whose complementary scientific and technological capabilities collectively form a single and unique European Research Infrastructure (RI) for the science of matter and advanced materials at the nanoscale, with upscale to micro analysis and technology. While maintaining the distributed nature of its constituent facilities, the IDRIN is designed to operate as a single integrated RI. Its operational model combines geographically distributed installations with central coordination mechanisms and local operational nodes, enabling harmonised access procedures, coordinated service delivery, and effective interoperability among providers. Through this approach, users can access via a Single Entry Point (SEP) a broad portfolio of techniques, instruments and expertise through a common framework, independently of the location of the resources required to perform their research. The coordinated operation of IDRIN relies on several dedicated components, each addressing a specific aspect of the infrastructure management. Scientific quality is ensured through the Access Review Panel (ARP), an independent external peer-review body responsible for evaluating the scientific merit of user proposals. Technical coordination is ensured by the Technical Liaison Network (TLNet), a distributed network composed of a central coordinating node and local nodes at provider sites, assessing technical feasibility, supporting users, and coordinating the implementation of optimised work plans across co-located and distributed facilities. The user-oriented dimension of the infrastructure is supported by the User Office Network (UONet) that coordinates the activities of local User Offices, aiming at the provision of harmonised administrative and logistical services throughout the access lifecycle. Through common procedures and shared service standards, UONet supports users in matters such as travel and subsistence reimbursement, logistics, legal and insurance aspects, while contributing to the integration of administrative information across the infrastructure. A further fundamental component of IDRIN is the data management framework, which supports the implementation of FAIR data principles and promotes advanced data stewardship practices across all infrastructure activities. The data management platform provides an integrated environment linking scientific datasets, metadata and access-related information, enabling the production, storage and exploitation of FAIR research data while supporting monitoring, reporting and future socio-economic analyses of the infrastructure usage. Together, these coordination components provide the scientific, technical, operational and datamanagement foundations required to operate the IDRIN as a coherent distributed infrastructure. To support the development of sustainable schemes for the long-term technical and scientific management of the IDRIN, the coordinators of the main operational components were invited to assess the strengths and weaknesses of the current operational model and explain their view on the needs for its further evolution. The analysis presented in this deliverable is based on their contributions and on the common themes emerging across the different coordination areas.
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WP2 - MGT2 - Pilot scheme for the management of a distributed research infrastructure offering harmonised, interoperable and integrated services
D2.8 - Second call for additional providers
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According to the Grant Agreement, in the lifetime of NFFA-Europe Pilot the Transnational Access offer must enlarge to meet (i) the qualitative needs of users that could be better met with new specialized providers, or (ii) quantitative needs resulting in oversubscription of the current capacity. To this aim, two calls for additional access providers were foreseen at M24 and M40, respectively. This report describes the rationale that led to the text of the second call for additional access providers, i.e. from the evaluation of the needs – mainly based on the analysis provided in the deliverable D2.7 “Second balance of access provision” - to the search for alternative solutions to widen and strengthen the current offer.
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WP14 - JA4 - A safe-by-design platform for nanomaterials
D14.4 - Integration and characterization of the space correlation functionality on the complete setup
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This report presents an experimental workflow designed to perform correlative measurements as part of the NEP-NFFA project, using silicon nitride (Si3N4) membranes equipped with platinum (Pt) markers. These membranes were developed through a collaboration between DESY NanoLab and ESRF-ID21. DESY NanoLab was responsible for the Pt deposition on the Si3N4 membranes, while ESRF-ID21 carried out the correlative measurements. The purpose of the Pt markers on the membranes is to act as fiducial points that help to precisely locate specific regions or points of interest (ROI/POI) with micrometric or nanometric accuracy. This is essential to analyze the same point of the sample using different techniques, and to collect complementary data for a better understanding of the sample. The proposed workflow involves complementary techniques such as optical microscopy, scanning electron microscopy (SEM), and synchrotron-based techniques like micro X-ray fluorescence (µXRF) performed at the nano-X-ray microscope (nano-SXM) at beamline ID21 of the Softhis report describes the initial design of the membranes with the markers, as well as the optimized version, based on results obtained during the first tests with nano-SXM. In addition, it shows how the Pt markers enable accurate correlation through the web-based graphical interface Daiquiri, linking the morphological information from optical microscopy with the chemical information obtained from µXRF. The technical feasibility of this approach has been confirmed, showing that the process is reproducible and potentially applicable to similar studies. This is possible due to the standardized Pt deposition process and the use of nano-SXM for sample localization and data acquisition.
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Transnational Access Statistics
31 calls for access
1176 proposals submitted
61% rate of acceptance
30% with Large Scale Facilities
13% with theory
12% with industry
~3 average users per proposal
66 countries applying
3653 lab sessions